Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (132,619)

Singleplex and Multiplex Bead Based Immunoassays (6,174)

ELISA Substrates and Reagents (2,341)

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331 – 345 of 138,521 results

331

Invitrogen™ Mouse IgG2c Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Immunoassay Kit Format	Uncoated ELISA Kit with Plates
Target Species	Mouse
Conjugate	HRP
Total Assay Time	24 hr 30 min
Assay Sensitivity	0.39 ng/mL
Storage Requirements	2-8°C
Assay Range	0.39-25 ng/mL
Test Time	1 hr 45 min
Regulatory Status	RUO
Target	Mouse IgG2c
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Mouse IgG2c protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Mouse IgG2c protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

332

Invitrogen™ Human STAT3 (Total/Phospho) InstantOne™ ELISA Kit

ELISA

Pricing & Availability
Specifications

Protein Aliases	Acute-phase response factor
Immunoassay Kit Format	Coated ELISA Kit
Target Species	Human,Human
Conjugate	HRP
Gene Symbol	STAT3
Total Assay Time	1 hr 10 min
Storage Requirements	2-8°C
Test Time	1 hr 20 min
Accession Number	P40763
Regulatory Status	RUO
Target	STAT3
Kit Contents	Capture Antibody, Detection Antibody Reagent, InstantOneTM Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal,Detection Antibody Reagent,InstantOne™ Assay Plate,Cell Lysis Buffer,Enhancer Solution,Wash Buffer (10X),Substrate Reagent (TMB),Stop Solution,Positive Control Cell Lysate (lyophilized),Plate seal
Gene Alias	1110034C02Rik; acute phase response factor; acute-phase response factor; ADMIO; ADMIO1; APRF; AW109958; DNA-binding protein APRF; FLJ20882; HIES; MGC16063; signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); signal transducer; activator of transcription; acute-phase response factor; signal transduction and activation of transcription 3; STAT; STAT3; STAT-3; stat3 protein; STAT3b1; STAT3b2; transcription factor; Unknown (protein for MGC:128731); wu:fc15d02; wu:fl59g06; z-Stat31110034C02Rik; acute phase response factor; acute-phase response factor; ADMIO; ADMIO1; APRF; AW109958; DNA-binding protein APRF; FLJ20882; HIES; MGC16063; signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); signal transducer; activator of transcription; acute-phase response factor; signal transduction and activation of transcription 3; STAT; STAT3; STAT-3; stat3 protein; STAT3b1; STAT3b2; transcription factor; Unknown (protein for MGC:128731); wu:fc15d02; wu:fl59g06; z-Stat3; ADMIO; ADMIO1; APRF; HIES
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	6774
Sample Type	Cell Lysates
Label or Dye	Biotin

333

Invitrogen™ Human C1q ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Intraassay CV	4.7%
Total Assay Time	4 hr 30 min
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity	0.08 ng/mL
Assay Range	0.9-60 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	C1q
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Lysis Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	712, 713, 714
Sample Type	Plasma,Serum,Supernatant
Synonym	complement component 1, q subcomponent, A chain el, complement component 1, q subcomponent, alpha poly

334

Invitrogen™ Mouse IFN gamma ProQuantum Immunoassay Kit

ELISA

Pricing & Availability
Specifications

Immunoassay Kit Format	Proximity-based Immunoassay
Target Species	Mouse
Conjugate	FAM
Incubation Time	1 hr.
Gene Symbol	IFNG
Total Assay Time	2 hr
Sample Volume	Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity	<0.05 pg/mL
Storage Requirements	-20°C
Assay Range	0.064-5000 pg/mL
For Use With (Application)	Protein Quantitation
Test Time	30 min
Detection Method	Taqman™ Real-time PCR
Accession Number	P01580
Regulatory Status	RUO
Target	IFN gamma
Kit Contents	Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment)	qPCR Instruments
Product Type	ProQuantum
Gene ID (Entrez)	15978
Sample Type	Plasma,Serum,Supernatant
Label or Dye	FAM™

335

Invitrogen™ Human IL-18 ProQuantum Immunoassay Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Immunoassay Kit Format	Proximity-based Immunoassay
Target Species	Human
Conjugate	FAM
Gene Symbol	IL18
Total Assay Time	2 hr
Assay Sensitivity	<0.05 pg/mL
Storage Requirements	-20°C
Assay Range	0.064-1000 pg/mL
Test Time	30 min
Accession Number	Q14116
Regulatory Status	RUO
Target	IL-18
Kit Contents	Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
Gene Alias	cytokine; iboctadekin; IFN-gamma-inducing factor; IGIF; il 18; IL-1 gamma; IL18; IL-18; IL1F4; IL-1g; ILN; interferon gamma inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; Interleukin18; Interleukin-18; interleukin-18 proprotein; interleukin-18-like protein; MGC12320cytokine; iboctadekin; IFN-gamma-inducing factor; IGIF; il 18; IL-1 gamma; IL18; IL-18; IL1F4; IL-1g; ILN; interferon gamma inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; Interleukin18; Interleukin-18; interleukin-18 proprotein; interleukin-18-like protein; MGC12320;
For Use With (Equipment)	qPCR Instruments
Product Type	ProQuantum
Gene ID (Entrez)	3606
Sample Type	Plasma,Serum,Supernatant

336

Invitrogen™ Human IFN gamma ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	5.7%
Intraassay CV	4.5%
Total Assay Time	3 hr 10 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.99 pg/mL
Storage Requirements	2-8°C
Assay Range	1.6-100 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	P01579
Regulatory Status	RUO
Target	IFN gamma
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3458
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Immune interferon, IFG

337

Invitrogen™ Granzyme B Monoclonal Antibody (N4TL33), Brilliant Violet™ 480, eBioscience™

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	4°C, store in dark, DO NOT FREEZE!
Target Species	Human
Host Species	Mouse
Conjugate	Brilliant Violet 480
Applications	Flow Cytometry
Form	Liquid
Gene Accession No.	P10144
Isotype	IgG2a κ
Concentration	5 μL/Test
Antigen	Granzyme B
Gene Symbols	GZMB
Regulatory Status	RUO
Purification Method	Affinity chromatography
Gene Alias	AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E
Gene	GZMB
Product Type	Antibody
Gene ID (Entrez)	3002
Formulation	PBS with BSA and 0.09% sodium azide; pH 7.2
Classification	Monoclonal
Primary or Secondary	Primary
Clone	N4TL33

338

Invitrogen™ Chicken IL-12 p40 ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	Il12b
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	9 pg/mL
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	9-2000 pg/mL
Test Time	1 hr 20 min
Accession Number	CLMF; CLMF p35; CLMF p40; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; Il12; IL-12 p40 subunit; IL-12 subunit p35; IL-12 subunit p40; IL12, subunit p40; IL-12.p40; IL12A; IL-12 A; Il12b; IL-12 B; Il12p40; Il-12p40; ILN; IMD28; IMD29; Interleukin; Interleukin 12; interleukin 12 p40 subunit; interleukin 12, p40; interleukin 12 B; interleukin 12 B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); Interleukin12; interleukin-12 beta chain; interleukin-12 p40; interleukin-12 p40 chain; interleukin-12 p40 subunit; interleukin-12 subunit beta; interleukin-12p40; interleukin-23 p40 subunit; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; p40; RP23-388G23.1
Regulatory Status	RUO
Target	IL-12 p40
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	404671
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

339

Invitrogen™ ProcartaPlex™ Canine Cytokine/Chemokine/Growth Factor Panel 1, 11plex

The ProcartaPlex Canine Cytokine/Chemokine/Growth Factor Panel 1 11plex enables the study of immune function by analyzing 11 protein targets in a single well using Luminex xMAP technology.

Pricing & Availability

340

Invitrogen™ Human IgM ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	3.2%
Intraassay CV	5.1%
Total Assay Time	1 hr 30 min
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity	1 ng/mL
Storage Requirements	2-8°C
Assay Range	15.6-1000 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	Human IgM
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3507
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Immunoglobulin M

341

Invitrogen™ Human IgG4 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Intraassay CV	4.6%
Total Assay Time	1 hr 30 min
Sample Volume	Plasma 20 μL, Serum 20 μL
Assay Sensitivity	0.1 ng/mL
Assay Range	9.4-600 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	Human IgG4
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3503
Sample Type	Plasma,Serum
Synonym	Immunoglobulin G4

342

Invitrogen™ Human Complement C5a ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Intraassay CV	3.6%
Total Assay Time	4 hr 30 min
Sample Volume	Plasma 10 μL, Serum 5 μL, Supernatant 50 μL
Assay Sensitivity	0.005 ng/mL
Assay Range	0.078-5.0 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	Complement C5a
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	727
Sample Type	Plasma,Serum,Supernatant
Synonym	complement component 5, C5a, C5D, C5b, ECLZB, CPAMD4

343

Invitrogen™ Human IL-10 Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	IL10
Total Assay Time	24 hr 30 min
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	4.7 pg/mL
Storage Requirements	2-8°C
Assay Range	4.7-300 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	P22301
Regulatory Status	RUO
Target	IL-10
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3586
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Interleukin-10

344

Invitrogen™ Human TGF beta-1 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	4.9%
Intraassay CV	3.2%
Total Assay Time	4 hr 30 min
Sample Volume	Plasma 20 μL, Serum 20 μL, Supernatant 20 μL
Assay Sensitivity	8.6 pg/mL
Storage Requirements	2-8°C
Assay Range	31-2000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	P01137
Regulatory Status	RUO
Target	TGF beta-1
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7040
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Camurati-Engelmann disease, DPD1

345

Invitrogen™ Human HSP70 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Intraassay CV	5.3%
Total Assay Time	4 hr
Sample Volume	Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.052 ng/mL
Assay Range	0.156-10.0 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	HSPA4
Kit Contents	Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3308
Sample Type	Cell Lysates,Plasma,Serum,Supernatant
Synonym	Heat Shock Protein 70

Immunoassay Reagents and Kits

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Invitrogen™ Mouse IFN gamma ProQuantum Immunoassay Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Mouse IFN gamma ProQuantum Immunoassay Kit